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L5 BIOL

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Paste a screenshot of the completed Procedure I data run below. Make sure you know how your computer/device saves screenshots. Follow
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Data Table I

Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)

4

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1

TableTop Science – All Rights Reserved

Leaf

Sample

Type

Illumination

Level

Oxygen

Concentration

Change Data (ppm)

Elapsed

Time

(s)

Oxygen

Change Rate

(ppm/s)

Sun-

Adapted

100%

Shade-Adapted

100%

Leaf

Sample

Type

Illumination

Level

Carbon Dioxide

Concentration

Change Data

(ppm)

Elapsed

Time

(s)

Carbon Dioxide

Change Rate

(ppm/s)

Sun-

Adapted

100%

Shade-Adapted

100%

Observations and Questions

[1] What is the function of chlorophyll in photosynthesis?

[2] What are enzymes? What is their function in biological and chemical reactions? Specifically, what do they do to a chemical reaction’s activation energy?

[3] How might enzyme concentration differences affect rates of photosynthesis and respiration?

Answer

[4] Show your oxygen change rate calculation for the Sun-Adapted data run. Be sure to include the units of measurement for each step.

Calculation

[5] Show your carbon dioxide change rate calculation for the Shade-Adapted data run. Be sure to include the units of measurement for each step.

Calculation

[6] (a) What is the chemical reaction for photosynthesis?

Answer (a)

(b) Carefully examine your results from Procedure I. Do your results support the relationship between oxygen and carbon dioxide shown in the chemical reaction? Explain and cite your results as part of your explanation.

[7] In procedure I, the oxygen concentration changes differed between sun-adapted leaves and shade adapted leaves. Can chlorophyll concentrations alone explain the differences seen between the two leaf types? Explain your answer, citing your results to support this.

[8] What is the difference between photosynthesis rate and net photosynthesis rate? Which rate are you determining in this procedure? Explain.

0%? Explain why (or why not) you would expect this?

Answer

Procedure II – Respiration Rates – 0% Illumination Data Run

Data Table II

Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)

2

TableTop Science – All Rights Reserved

3

TableTop Science – All Rights Reserved

Leaf

Sample

Type

Illumination

Level

Oxygen

Concentration

Change Data (ppm)

Elapsed

Time

(s)

Oxygen

Change Rate

(ppm/s)

Sun-

Adapted

0%

Shade-Adapted

0%

Leaf

Sample

Type

Illumination

Level

Carbon Dioxide

Concentration

Change Data (ppm)

Elapsed

Time

(s)

Carbon Dioxide

Change Rate

(ppm/s)

Sun-

Adapted

0%

Shade-Adapted

0%

Observations and Questions

[10] What would be the photosynthesis rate if measured at 0% illumination? Explain your answer. Cite your results from Procedure II to assist with your explanation.

[11] Using your results for the change in carbon dioxide concentration in Procedure II as a reference, compare the rates of cellular respiration for sun-adapted versus shade-adapted leaves. Explain the biological principles/bases underlying this comparison.

Procedure III – Low-Light Net Photosynthesis Rates – 15% Illumination

Data Table III

Complete the table below using your data and information found under the Background tab (see the Summary of Needed Formulas section)

Leaf

Sample

Type

Illumination

Level

Oxygen

Concentration

Change Data (ppm)

Elapsed

Time

(s)

Oxygen

Change Rate

(ppm/s)

Sun-

Adapted

15%

Shade-Adapted

15%

4

TableTop Science – All Rights Reserved

5

TableTop Science – All Rights Reserved

Observations and Questions

[12] Based on the data you collected in Procedures I (100% illumination) and III (15% illumination), which system of leaves produces the most oxygen: Sun-adapted or shade-adapted? Is one system (sun- or shade-adapted) more productive than the other in all light levels? Explain how your results support your answer.

Answer :

[13] Are your results from Procedures II and III consistent with research findings that there are differences in chlorophyll production and enzyme activity between shade-adapted leaves and sun-adapted leaves? Explain your reasoning. Cite your results from Procedure II and Procedure III to support your explanation.

Screenshots

Procedure 1

Sun- dapted leaf trial

Before the trial

Shade-adapted trial

Before trial

After

Procedure 2

Before

Set to 0% illumination

Before the shade

Adjusted to 0%

Procedure 3

Set it up to 15% illumination

Set up shade to 15%

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