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Data Table I
Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)
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|
Leaf Sample Type |
Illumination Level |
Oxygen Concentration Change Data (ppm) |
Elapsed Time (s) |
Oxygen Change Rate (ppm/s) |
|
Sun- Adapted |
100% |
|||
|
Shade-Adapted |
100% |
|
Leaf Sample Type |
Illumination Level |
Carbon Dioxide Concentration Change Data (ppm) |
Elapsed Time (s) |
Carbon Dioxide Change Rate (ppm/s) |
|
Sun- Adapted |
100% |
|||
|
Shade-Adapted |
100% |
Observations and Questions
[1] What is the function of chlorophyll in photosynthesis?
[2] What are enzymes? What is their function in biological and chemical reactions? Specifically, what do they do to a chemical reaction’s activation energy?
[3] How might enzyme concentration differences affect rates of photosynthesis and respiration?
Answer
[4] Show your oxygen change rate calculation for the Sun-Adapted data run. Be sure to include the units of measurement for each step.
Calculation
[5] Show your carbon dioxide change rate calculation for the Shade-Adapted data run. Be sure to include the units of measurement for each step.
Calculation
[6] (a) What is the chemical reaction for photosynthesis?
Answer (a)
(b) Carefully examine your results from Procedure I. Do your results support the relationship between oxygen and carbon dioxide shown in the chemical reaction? Explain and cite your results as part of your explanation.
[7] In procedure I, the oxygen concentration changes differed between sun-adapted leaves and shade adapted leaves. Can chlorophyll concentrations alone explain the differences seen between the two leaf types? Explain your answer, citing your results to support this.
[8] What is the difference between photosynthesis rate and net photosynthesis rate? Which rate are you determining in this procedure? Explain.
0%? Explain why (or why not) you would expect this?
Answer
Procedure II – Respiration Rates – 0% Illumination Data Run
Data Table II
Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)
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|
Leaf Sample Type |
Illumination Level |
Oxygen Concentration Change Data (ppm) |
Elapsed Time (s) |
Oxygen Change Rate (ppm/s) |
|
Sun- Adapted |
0% |
|||
|
Shade-Adapted |
0% |
|
Leaf Sample Type |
Illumination Level |
Carbon Dioxide Concentration Change Data (ppm) |
Elapsed Time (s) |
Carbon Dioxide Change Rate (ppm/s) |
|
Sun- Adapted |
0% |
|||
|
Shade-Adapted |
0% |
Observations and Questions
[10] What would be the photosynthesis rate if measured at 0% illumination? Explain your answer. Cite your results from Procedure II to assist with your explanation.
[11] Using your results for the change in carbon dioxide concentration in Procedure II as a reference, compare the rates of cellular respiration for sun-adapted versus shade-adapted leaves. Explain the biological principles/bases underlying this comparison.
Procedure III – Low-Light Net Photosynthesis Rates – 15% Illumination
Data Table III
Complete the table below using your data and information found under the Background tab (see the Summary of Needed Formulas section)
|
Leaf Sample Type |
Illumination Level |
Oxygen Concentration Change Data (ppm) |
Elapsed Time (s) |
Oxygen Change Rate (ppm/s) |
|
Sun- Adapted |
15% |
|||
|
Shade-Adapted |
15% |
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Observations and Questions
[12] Based on the data you collected in Procedures I (100% illumination) and III (15% illumination), which system of leaves produces the most oxygen: Sun-adapted or shade-adapted? Is one system (sun- or shade-adapted) more productive than the other in all light levels? Explain how your results support your answer.
Answer :
[13] Are your results from Procedures II and III consistent with research findings that there are differences in chlorophyll production and enzyme activity between shade-adapted leaves and sun-adapted leaves? Explain your reasoning. Cite your results from Procedure II and Procedure III to support your explanation.
Screenshots
Procedure 1
Sun- dapted leaf trial
Before the trial
Shade-adapted trial
Before trial
After
Procedure 2
Before
Set to 0% illumination
Before the shade
Adjusted to 0%
Procedure 3
Set it up to 15% illumination
Set up shade to 15%
